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ATCC30788 stock 317
基本信息
资源编号 ATCC30788
资源名称 stock 317
种属 Paramecium novaurelia Sonneborn
分离基物 Cernay, France, pre-1958
提供形式 test tube
安全等级 1
模式菌株 no
培养方法
培养基 Medium 802: Sonneborn's Paramecium medium
传代方法 Protocol: ATCCNO: 30300 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, and loosen the cap one half turn. Add 1.0 ml of ATCC medium 802 bacterized with Enterobacter aerogenes ATCC 13048 twice weekly. When the tube is filled to within one inch of the top, decant leaving 5.0 ml in the original tube. Subcultures are established by transferring 0.5 ml of a growing culture to 5.0 ml of bacterized ATCC medium 802 in a 20 x 120 mm test tube.
生长条件 Max Temperature: 31.0°C
Min Temperature: 13.0°C
Protocol: ATCCNO: 30300 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, and loosen the cap one half turn. Add 1.0 ml of ATCC medium 802 bacterized with Enterobacter aerogenes ATCC 13048 twice weekly. When the tube is filled to within one inch of the top, decant leaving 5.0 ml in the original tube. Subcultures are established by transferring 0.5 ml of a growing culture to 5.0 ml of bacterized ATCC medium 802 in a 20 x 120 mm test tube.
存储条件 Cryoprotective SolutionDMSO 1.5 mlFresh growth medium w/o bacteria 7.5 mlMgCl2 (0.5 mM) 0.5 mlCaCl2 (0.5 mM) 0.5 ml1. Mix the components in the order listed. Before adding the MgCl2 and the CaCl2 allow the solution to return to room temperature. When the medium is added to the DMSO the solution will warm up due to chemical heat. 2. Harvest cells from a culture that is at or near peak density by filtration and centrifugation at 200 x g for 1 min.3. Adjust the concentration of cells to 2 x 105/ml in fresh medium.4. Mix the cell preparation and the cryoprotective solution in equal portions. 5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). 6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. 7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.8. To establish a culture from the frozen state add 1.0 ml ATCC medium 802 to the frozen ampule and place it in a 35°C water bath. Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial. 9.   Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate onto the surface of an ATCC medium 919 (non-nutrient agar) plate containing an overlay of 15.0 ml of bacterized ATCC medium 802.10. Incubate at 25°C.11. Once the culture is established, transfer 0.5 ml to 5.0 ml of bacterized ATCC medium 802.12. Follow the protocol for maintenance of culture.
详细说明
Year of Origin 1958