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32D Clone 3-菌种及细胞-北纳生物

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基本信息
资源编号 BNCC242235
资源名称 32D Clone 3
种属 Mus musculus, mouse│bone marrow
形态 lymphoblast
提供形式 frozen
安全等级 1
应用领域 This cell line is a suitable transfection host.
培养方法
培养基 RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with: 10% heat-inactivated fetal bovine serum 10% mouse Interleukin-3 culture supplement (available from Becton Dickinson Cat. No. 354040, formerly Collaborative Research Cat. No. 40040)
传代方法 Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 105 viable cells/mL. Maintain cell density between 2 X 105 and 1 X 106 viable cells/mL. Scrape off the attached cells and transfer along with the floating cells into new flasks.<br>Medium Renewal: Every 2 to 3 days
生长条件 Temperature: 37&deg;C
生长特性 mixed, adherent and suspension
存储条件 liquid nitrogen vapor temperature;Freeze medium: Complete growth medium 95%; DMSO, 5%<br>Storage temperature: liquid nitrogen vapor temperature
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