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NK-92-菌种及细胞-北纳生物

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基本信息
资源编号 BNCC236019
资源名称 NK-92
种属 Homo sapiens, human│peripheral blood
类型 natural killer cell; NK cell
形态 lymphoblast
提供形式 frozen
安全等级 2
模式菌株 未知
应用领域 This cell line is a suitable transfection host.The cell line is cytotoxic to a wide range of malignant cells; it kills both K562 cells and Daudi cells in chromium release assays.NK-92 cells (after irradiation to prevent proliferation) can be used effectively for immunological ex vivo purging of leukemia from blood without compromising hematopoietic cell function.
培养方法
培养基 The base medium for this cell line is Alpha Minimum Essential medium without ribonucleosides and deoxyribonucleosides but with 2 mM L-glutamine and 1.5 g/L sodium bicarbonate . To make the complete growth medium, add the following components to the base medium: 0.2 mM inositol; 0.1 mM 2-mercaptoethanol; 0.02 mM folic acid; 100-200 U/ml recombinant IL-2; adjust to a final concentration of 12.5% horse serum and 12.5% fetal bovine serum.
传代方法 Cultures can be maintained by addition or replacement of medium. When replacing media, centrifuge cells and resuspend cell pellet in fresh medium at 2 to 3 X 105 viable cells/mL. Pipet the cells up and down on the back of the flask every 2-3 days to produce a single cell suspension. Corning&reg; T-75 flasks (catalog #431464) are recommended for subculturing this product. NK-92 cells are extremely sensitive to overgrowth and media exhaustion.<br>Medium Renewal: Replace with fresh medium every 2 to 3 days (depending on cell density)
生长条件 Atmosphere: air, 95%; carbon dioxide (CO2), 5%<br>Temperature: 37°C<br>Growth Conditions: Successful growth of this cell line is very dependent upon the quality of IL-2 used in the growth medium. ATCC recommends using the highest quality IL-2 available.
生长特性 suspension, multicell aggregates
存储条件 liquid nitrogen vapor phase;Freeze medium: 50% FBS; 40% complete growth medium ; 10% DMSO.<br>Storage temperature: liquid nitrogen vapor phase
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