| 资源编号 | BNCC233051 |
| 资源名称 | AML12 |
| 种属 | Mus musculus, mouse│liver |
| 类型 | hepatocyte |
| 形态 | epithelial |
| 提供形式 | frozen |
| 安全等级 | 1 |
| 应用领域 | This cell line is a suitable transfection host. |
| 培养基 | A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 0.005 mg/ml insulin, 0.005 mg/ml transferrin, 5 ng/ml selenium, and 40 ng/ml dexamethasone, 90%; fetal bovine serum, 10% |
| 传代方法 | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:4 to 1:6 Medium Renewal: 2 to 3 times a week. <br>Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| 生长条件 | Temperature: 37°C |
| 生长特性 | adherent |
| 存储条件 | liquid nitrogen vapor phase;Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO<br>Storage temperature: liquid nitrogen vapor phase |
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