| 资源编号 | BNCC290808 |
| 资源名称 | A549 |
| 种属 | Homo sapiens, human│lung |
| 形态 | epithelial |
| 提供形式 | frozen |
| 安全等级 | 1 |
| 应用领域 | This cell line is a suitable transfection host. |
| 培养基 | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| 传代方法 | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Cultures can be established between 2 x 103 and 1 x 104 viable cells/cm2. Do not exceed 7 x 104 cels/cm2. Incubate cultures at 37°C. Interval: Maintain cultures at a cell concentration between 6 X 103 and 6 X 104 cell/cm2.<br>Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended<br>Medium Renewal: 2 to 3 times per week |
| 生长条件 | Atmosphere: air, 95%; carbon dioxide (CO2), 5%<br>Temperature: 37°C |
| 生长特性 | adherent |
| 存储条件 | liquid nitrogen vapor phase;Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO<br>Storage temperature: liquid nitrogen vapor phase |
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