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THP-1-菌种及细胞-北纳生物

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基本信息
资源编号 BNCC288554
资源名称 THP-1
种属 Homo sapiens, human│peripheral blood
类型 monocyte
形态 monocyte
提供形式 frozen
安全等级 1
应用领域 These cells are suitable as a transfection host.
培养方法
培养基 The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
传代方法 Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2-4 x 105 viable cells/mL. Subculture when cell concentration reaches 8x105 cells/mL.Do not allow the cell concentration to exceed 1 x 106 cells/mL.&nbsp;Corning&reg; T-75 flasks (catalog #431464) are recommended for subculturing this product.<br>Medium Renewal: Every 2 to 3 days
生长条件 Atmosphere: air, 95%; carbon dioxide (CO2), 5%<br>Temperature: 37.0&deg;C
生长特性 suspension
存储条件 liquid nitrogen vapor phase;Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO<br>Storage temperature: liquid nitrogen vapor phase
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