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Sf9-菌种及细胞-北纳生物

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基本信息
资源编号 BNCC271491
资源名称 Sf9
种属 Spodoptera frugiperda, fall armyworm│ovary
形态 epithelial
提供形式 frozen
安全等级 1
应用领域 This line can be used to replicate baculovirus expression vectors. This cell line is a suitable transfection host.
培养方法
培养基 The base medium for this cell line is Grace's Insect Medium Supplemented (GIBCO/Invitrogen Cat. No. 11605-094 or equivalent). To make the complete growth medium, add the following components to the base medium: heat-inactivated insect cell culture tested fetal bovine serum to a final concentration of 10%.
传代方法 Gently resuspend cells in the spent culture medium by pipetting across the monolayer or by hitting the flask against the palm of your hand (the latter is only preferable when working with larger flasks).<br>Subcultivation Ratio: A subcultivation ratio of 1:5 or greater is recommended<br>Medium Renewal: Every 2 to 3 days
生长条件 Atmosphere: air, 100%<br>Temperature: 28&deg;C<br>Growth Conditions: The recommended media are formulated for use without CO2. Omission of the yeastolate or lactalbumin hydrolysate will lead to poor performance by this line.
生长特性 mixed: adherent/suspension
存储条件 liquid nitrogen vapor phase;Freeze medium: culture medium, 95%; DMSO, 5%<br>Storage temperature: liquid nitrogen vapor phase
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