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HUV-EC-C [HUVEC]-菌种及细胞-北纳生物

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基本信息
资源编号 BNCC249736
资源名称 HUV-EC-C [HUVEC]
种属 Homo sapiens, human│umbilical vein/vascular endothelium
类型 endothelial
形态 endothelial
提供形式 frozen
安全等级 1
应用领域 This cell line is a suitable transfection host.
培养方法
培养基 The base medium for this cell line is ATCC-formulated of F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.1 mg/ml heparin; 0.03-0.05 mg/ml endothelial cell growth supplement (ECGS); adjust to a final concentration of 10% fetal bovine serum.
传代方法 Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.&nbsp;Corning&reg; T-75 flasks (catalog #430641) are recommended for subculturing this product. Note: A high quality ECGS prepared from bovine neural tissue (Sigma Cat no. E-2759 or equivalent) should be used to propagate CRL-1730. It is best to initiate the cells with the highest recommended concentration of ECGS.&nbsp; Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37&deg;C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37&deg;C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended<br>Medium Renewal: Two to three times per week
生长条件 Atmosphere: air, 95%; carbon dioxide (CO2), 5%<br>Temperature: 37&deg;C
生长特性 adherent
存储条件 liquid nitrogen vapor phase;Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO<br>Storage temperature: liquid nitrogen vapor phase
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