| 资源编号 | BNCC246354 |
| 资源名称 | HeLa |
| 种属 | Homo sapiens, human│cervix |
| 类型 | epithelial |
| 形态 | epithelial |
| 提供形式 | frozen |
| 安全等级 | 2 |
| 应用领域 | These cells are a suitable transfection host.<br>This cell line can be used to screen for Escherichia coli strains with invasive potential. |
| 培养基 | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| 传代方法 | Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. <br>Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended<br>Medium Renewal: 2 to 3 times per week |
| 生长条件 | Atmosphere: Air, 95%; carbon dioxide (CO2), 5%<br>Temperature: 37°C |
| 生长特性 | adherent |
| 存储条件 | liquid nitrogen vapor phase;Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO<br>Storage Temperature: Liquid nitrogen vapor phase |
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