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TeloHAEC-菌种及细胞-北纳生物

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基本信息
资源编号 BNCC262802
资源名称 TeloHAEC
种属 Homo sapiens, human│aorta
类型 endothelial
形态 endothelial
提供形式 frozen
安全等级 2
培养方法
培养基 The base medium for this cell line is Vascular Cell Basal Medium (ATCC PCS-100-030), supplemented with Vascular Endothelial Cell Growth Kit-VEGF (ATCC PCS-100-041).   Optional: Add 0.3ug/mL Puromycin(Santa Cruz Biotech: sc-108071A). Note: Do not filter complete medium.
传代方法 Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes. Subculture when the culture is about 90% confluent. Remove and discard spent medium. Briefly rinse the cells with Dulbecco's Phosphate Buffered Saline (DPBS, ATCC 30-2200) and discard rinse solution. Add 2.0 to 3.0 mL room temperature Trypsin-EDTA for Primary Cells (ATCC PCS-999-003) to the flask. Incubate at 37°C for 5 min (until cells have detached). Neutralize trypsin by adding an equal volume of room temperature 2% FBS in DPBS. Centrifuge cells at 250 x g for 5 min at room temperature. Remove supernatant. Resuspend pellet in 6.0 to 8.0 mL Complete Growth Medium. Count cells, and seed 5.0 x 103 to 8.0 x 103 viable cells/cm2 to new culture vessels. Medium Renewal: Every 2-3 days.
生长条件 Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C
生长特性 adherent
存储条件 liquid nitrogen vapor phase;90% FBS, 10% DMSO
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